Development System Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea | SCGE Toolkit LNP delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea (inner ear)
SCGE Toolkit downloaded on: 2023/03/22 11:45:30; Please cite the Somatic Cell Genome Editing Consortium Toolkit NIH HG010423 when using publicly accessible data in formal presentation or publication. SCGE Experment ID: 18000000023. PI:
Zheng-Yi Chen PhD
LNP delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea (inner ear)
In vitro screening of LNPs. Primary fibroblast cells were derived from skins of P5 Ai14 mice and plated in 48-well plates at 10,000-20,000 cells per well. LNP was mixed with Cas9 mRNA and sgRNA in 15:1:1 ratio and added to the well. The final concentration of LNP is 7.6 ug/ml. Cells were collected 48 hr later to check tdT signal. a, 306-O12B; b, 306-S10