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Experiment: Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea

PI:   Zheng-Yi Chen, PhD 
 
 
LNP delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea (inner ear)
Editing Assay: - Editing is displayed by tdTomato signal 48 hr after transfection.

Filter Results ..
Editors
 CleanCap® Cas9
Guide Target Locus
 Ai9/Ai14 reporter transgene
Guides
 sg298
Models
 Ai14 mouse primary fibroblasts

Results
Testing_new_LNPs_(lipid_nanoparticles)_for_delivery_of_Cas9_mRNA/sgRNA_in_primary_fibroblast_cells_from_adult_Ai14_mouse_cochlea
Editing Efficiency
Record Id Condition Editor Model Delivery Target Locus Guide Dosage Editing Efficiency Signal Image
15000000589 306-O12B CleanCap® Cas9 Ai14 mouse primary fibroblasts 306-O12B Ai9/Ai14 reporter transgene sg298 LNP/Cas9/sgRNA=15/1/1, [LNP]=7.6ug/ml
15000000590 306-S10 CleanCap® Cas9 Ai14 mouse primary fibroblasts 306-S10 Ai9/Ai14 reporter transgene sg298 LNP/Cas9/sgRNA=15/1/1, [LNP]=7.6ug/ml
LNP delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea (inner ear)

In vitro screening of LNPs. Primary fibroblast cells were derived from skins of P5 Ai14 mice and plated in 48-well plates at 10,000-20,000 cells per well. LNP was mixed with Cas9 mRNA and sgRNA in 15:1:1 ratio and added to the well. The final concentration of LNP is 7.6 ug/ml. Cells were collected 48 hr later to check tdT signal. a, 306-O12B; b, 306-S10