Experiment: Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice
PI: Zheng-Yi Chen, PhD
Description: Delivery of firefly luciferase mRNA via new Lipid NanoParticles by tail vein injection into WT C57BL/6J mice targeting the Liver and delivery is measured by luciferase expression.
Delivery Assays:
Delivery efficiency was calculated by whole body luciferase expression
Parent Project: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles
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Other experiments in this project: 3
- Verification of new LNP 306-O12B for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea. Procedure performed side by side with JAX.
- Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea
- Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Download: Submitted files
Results |
| Delivery Efficiency | ||||||||
|---|---|---|---|---|---|---|---|---|
| Condition | Tissue | Editor | Model | Delivery | Dosage | Injection Frequency | Signal | Image |
| 306-O12B | liver | CleanCap® Fluc | C57BL/6J mouse | 306-O12B | 10 ug per mouse | once | ||
| 113-O12B | liver | CleanCap® Fluc | C57BL/6J mouse | 113-O12B | 10 ug per mouse | once | ||
| 306-S10 | liver | CleanCap® Fluc | C57BL/6J mouse | 306-S10 | 10 ug per mouse | once | ||
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In vivo luciferase assay for mRNA delivery. Selected LNPs were mixed with Fluc mRNA and injected into adult wild type mice via i.v. injection at 10 ug mRNA per mouse. The luciferase expression was evaluated 6 h later by live animal bioluminescence imaging. |
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