Experiment: Testing new LNPs (lipid nanoparticles) for delivery of Fluc mRNA in adult mice
PI: Zheng-Yi Chen, PhD
Description: Delivery of firefly luciferase mRNA via new Lipid NanoParticles by tail vein injection into WT C57BL/6J mice targeting the Liver and delivery is measured by luciferase expression.
Delivery Assays: Delivery efficiency was calculated by whole body luciferase expression
Parent Project: Efficient In Vivo RNP-Based Gene Editing in the Sensory Organ Inner Ear Using Bioreducible Lipid Nanoparticles
Other experiments in this project: 3
- Verification of new LNP 306-O12B for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea. Procedure performed side by side with JAX.
- Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in primary fibroblast cells from adult Ai14 mouse cochlea
- Testing new LNPs (lipid nanoparticles) for delivery of Cas9 mRNA/sgRNA in adult mouse cochlea
Download: Submitted files
Organ System Overview