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Tissues
Nervous
  • Brain
Editors
 SpyCas9-3xNLS
Guide Target Locus
 mTmG reporter transgene
Guides
 STS159 (mTmG; Sp_t2:Sp_c0_mTmG)
Delivery Systems
 S10
Models
 mTmG mouse (congenic)

Results

Delivery_of_unmodified,_phosphorothioate_(PS)-stabilized_crRNA_with_chemically_modified,_PS-stabilized_tracrRNA_using_the_S10_shuttle_peptide_to_activate_the_mTmG_reporter_in_mouse_brain_
Editing Efficiency
Record Id Condition Tissue Editor Model Delivery Target Locus Guide Dosage Injection Frequency Editing Efficiency Signal Image
15000000699 Sp_c0_mTmG/Sp_t2 + S10 shuttle peptide brain SpyCas9-3xNLS mTmG mouse (congenic) S10 mTmG reporter transgene STS159 (mTmG; Sp_t2:Sp_c0_mTmG) 8 µM RNP + 40 µM shuttle peptide S10 once