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Experiment: Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain

PI:   Erik J Sontheimer, PhD 

Chemically modified crRNA and tracrRNA were injected into the intra-striatum of mTmG reporter mice and activation of GFP expression was imaged.
Editing Assay: - Editing efficiency determined by immunohistochemical staining for GFP.

Organ System Overview
Delivery Efficiency
Editing Efficiency
Target Tissue
Not Studied

Sp_c0_mTmG/Sp_t2 + S10 shuttle peptide

Analyze Data Sets Available for this ExperimentDeliveryEditing
  Brain n/a