Experiment Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA using the S10 shuttle peptide to activate the mTmG reporter in mouse brain Experiment Condition Sp_c0_mTmG/Sp_t2 + S10 shuttle peptide Assay Description: Editing efficiency determined by immunohistochemical staining for GFP. Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000000699 Editor SpyCas9-3xNLS Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide STS159 (mTmG; Sp_t2:Sp_c0_mTmG) Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Sample Age 7-8 weeks Application Method Intra-Striatum Application Site brain Dosage 8 µM RNP + 40 µM shuttle peptide S10 Injection Frequency once Injection Rate 500 nl/min Injection Volume 3 microliters Days post injection 1 week Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Antibody RRID RRID:AB_1196615 Antibody Other ID AB_1196615 Antibody Description GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 2 of 2 present  1 present  2 present