Experiment Delivery of unmodified, phosphorothioate (PS)-stabilized crRNA with chemically modified, extended PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain Experiment Condition Sp_c0_mTmG/Sp_t2-5-3_PS-tail Assay Description: Editing efficiency determined by immunohistochemical staining for GFP. Tissue Measured: brain Measurement Type: Editing Efficiency Record ID 15000000700 Editor SpyCas9-3xNLS Delivery System Delivery System Subtype Guide STS159 (mTmG; Sp_t2-PS:Sp_c0_mTmG) Vector Model Species Mouse Model Name B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J Application Method Intra-Striatum Application Site brain Dosage 100 µM RNP Injection Frequency once Injection Rate 500 nl/min Injection Volume 2 microliters Days post injection 1 week Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Antibody RRID RRID:AB_1196615 Antibody Other ID AB_1196615 Antibody Description GFP (D5.1) XP Rabbit mAb antibody, Cell Signaling Technology Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 1 present and 1 present (weak)  1 present  2 present (weak)