Experiment Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Experiment Condition Control (Repeat 4/20/21): 306-O12B LNP without Cas9 mRNA and sgRNA Assay Description: Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei. Tissue Measured: base of cochlear canal Measurement Type: Editing Efficiency Record ID 15000001308 Editor Delivery System 306-O12B blank Delivery System Subtype Nanoparticle Guide Vector Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze/J Application Method canalostomy injection Application Site inner ear Dosage Injection Frequency once Injection Rate Injection Volume 1 microliter Days post injection 6 days Editor Format Lipid Nanoparticle Antidote Id Antidote Description Measured Values Samples Size: 2   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 2 of 2 absent  1 absent  2 absent