Experiment On-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver Experiment Condition OT8 (A4)-BE-eVLP Assay Description: adenine base editing at target Pcsk9 site in liver vs. other mouse organs (heart, kidney, lung, muscle, spleen) Tissue Measured: liver Measurement Type: Editing Efficiency Record ID 15000001540 Editor TadA-8e V106W Delivery System eVLP Delivery System Subtype Virus Like Particle Guide Vector Model Species Mouse Model Name C57BL/6J Application Method intravenous, retro-orbital Application Site liver Dosage 7E11 eVLPs Injection Frequency once Injection Rate Injection Volume 120 microliters Days post injection 1 week Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description Measured Values Samples Size: 4   Replicate Result (Editing Efficiency) Measurement Units: % of total sequencing reads with AT converted to GC Mean 0.01  1 0.023479  2 0.012232  3 0.013158  4 0