Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
ExperimentOn-target editing compared to 14 circle-seq nominated off-target sites of adenine base editor delivered by BE-eVLP vs AAV in the C57BL/6 liver
Experiment ConditionOT11 (A6)-BE-eVLP
Assay Description: adenine base editing at target Pcsk9 site in liver vs. other mouse organs (heart, kidney, lung, muscle, spleen)
Tissue Measured:liver
Measurement Type:Editing Efficiency
Record ID15000001543

EditorTadA-8e V106W
Delivery SystemeVLP
Delivery System SubtypeVirus Like Particle
Guide
Vector

Model SpeciesMouse
Model NameC57BL/6J

Application Methodintravenous, retro-orbital
Application Siteliver
Dosage7E11 eVLPs
Injection Frequencyonce
Injection Rate
Injection Volume120 microliters
Days post injection1 week
Editor Formatribonucleoprotein (RNP)
Antidote Id
Antidote Description

Measured Values
Samples Size: 4
 
Replicate Result (Editing Efficiency)
Measurement Units: % of total sequencing reads with AT converted to GC
Mean 0.01 
1 0.025041 
2 0.004222 
3 0.009606 
4 0.007518 
  

Title Description File Download SCGE ID
Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis This protocol describes in vivo adminstration and subsequent analysis of off-target editing in the liver. Chaikof-Associated Protocol 2_Off-target editing AND Primers for sequencing analysis.pdf 21000000033