Contact SCGE




SCGE Consortium Home   |  About SCGE Toolkit   |  Contact Us   |  Citing the Toolkit   |  License  
 
Filters ..
  Editors
 Alt-R A.s. Cas12a (Cpf1) V3
 Alt-RĀ® S.p. Cas9 Nuclease V3
 CleanCapĀ® Cre mRNA
 EnGenĀ®Ā Sau Cas9
Guide Target Locus
 GER14 (CAG-Venus*,-Katushka2S,-akaLuc)
  Guides
 GER14-g1-Cpf1-FWD
 GER14-g1-SaC9-FWD
 GER14-g1-SpC9-FWD
Models
 GER14 Heterozygous Blastocyst
Select experimental variable to highlight records on the chart: 
 

Note: Hover over the bars to view additional information

Loading...

Results

Validating_Gene_Editing_Reporter_14_(GER14)_Mouse_Model_in_Heterozygous_Blastocysts
Editing Efficiency
Record Id Condition Editor Model Target Locus Guide Editing Efficiency Signal Editing Efficiency % of blasts with RFP activation Editing Efficiency % of blasts with edits Image
15000003591 GER14-g1-SpC9-FWD Alt-RĀ® S.p. Cas9 Nuclease V3 GER14 Heterozygous Blastocyst GER14 (CAG-Venus*,-Katushka2S,-akaLuc) GER14-g1-SpC9-FWD
15000003592 GER14-g1-SaC9-FWD EnGenĀ®Ā Sau Cas9 GER14 Heterozygous Blastocyst GER14 (CAG-Venus*,-Katushka2S,-akaLuc) GER14-g1-SaC9-FWD
15000003593 Cre CleanCapĀ® Cre mRNA GER14 Heterozygous Blastocyst
15000003594 GER14-g1-Cpf1-FWD Alt-R A.s. Cas12a (Cpf1) V3 GER14 Heterozygous Blastocyst GER14 (CAG-Venus*,-Katushka2S,-akaLuc) GER14-g1-Cpf1-FWD

https://stage.scge.mcw.edu/toolkit/data/experiments/experiment/18000000075/record/15000003591/

Zygotes were electroporated with SpCas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Katushka2S (RFP) fluorescence and scored for fluorescence signal.

SaCas9 Validation of Gene Editing Reporter 14 (GER14) Mouse Model in Heterozygous Blastocysts

Zygotes were electroporated with SaCas9 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Katushka2S (RFP) fluorescence and scored for fluorescence signal.

Cpf1 Validation of Gene Editing Reporter 14 (GER14) Mouse Model in Heterozygous Blastocysts

Zygotes were electroporated with Cpf1 RNP along with guides and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Katushka2S (RFP) fluorescence and scored for fluorescence signal.

Cre Validation of Gene Editing Reporter 14 (GER14) Mouse Model in Heterozygous Blastocysts

Zygotes were electroporated with Cre mRNA and cultured 96 hours to blastocyst stage. Blastocysts were imaged for Katushka2S (RFP) fluorescence and scored for fluorescence signal.