Experiment Testing different ratios of Lipofectamine-RNPs after 24 hours for determination of positive control Experiment Condition RNP-NC Lipo 2:1 Assay Description: Calculated transfection efficiency by imaging fluorescent cells (Atto 550) compared to DAPI stained cells, and quantified using Image J. Tissue Measured: Measurement Type: Delivery Efficiency Record ID 15000003498 Editor sNLS-SpCas9-sNLS Delivery System RNP-NC-no ligand with Lipofectamine 2001 Delivery System Subtype Nanoparticle Guide Negative control gRNA Vector Model Species human Model Name Liver On Chip (LoC) Application Method chemical Application Site Dosage 2.4µg RNP Injection Frequency Injection Rate Injection Volume Days post injection 24 hours Editor Format Antidote Id Antidote Description Measured Values Samples Size: 1   Replicate Result (Delivery Efficiency) Measurement Units: Corrected total fluorescence (mean gray value) Mean 2000  1 2000.0