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Filters ..
Tissues
Nervous
  • Striatum  (TARGET)
Editors
 SpyCas9-3xNLS
  Guide Target Locus
 Dnmt1
 mTmG reporter transgene
  Guides
 STS159 (mTmG; Sp_t2:Sp_c20_mTmG)
 STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)
Models
 mTmG mouse (congenic)
  Sex
 Female
 Male
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Results

Independent_validation_of_Sontheimer_delivery_platform_using_heavily_modified_guide_RNAs_complexed_with_Cas9_proteins_to_deliver_CRISPR/Cas9_to_mouse_brain
Editing Efficiency
Record Id Condition Tissue Sex Editor Model Target Locus Guide Dosage Injection Frequency Editing Efficiency % edited cells in ROI Image
15000003052 DNMT1 C20/T2 SpyCas9 Males striatum (TARGET) Male SpyCas9-3xNLS mTmG mouse (congenic) Dnmt1 STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) 100 uM once
15000003053 DNMT1 C20/T2 SpyCas9 Females striatum (TARGET) Female SpyCas9-3xNLS mTmG mouse (congenic) Dnmt1 STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) 100 uM once
15000003054 mTmG c20/T2 SpyCas9 Males striatum (TARGET) Male SpyCas9-3xNLS mTmG mouse (congenic) mTmG reporter transgene STS159 (mTmG; Sp_t2:Sp_c20_mTmG) 100 uM once
15000003055 mTmG c20/T2 SpyCas9 Females striatum (TARGET) Female SpyCas9-3xNLS mTmG mouse (congenic) mTmG reporter transgene STS159 (mTmG; Sp_t2:Sp_c20_mTmG) 100 uM once

Title Description File Download SCGE ID
JAX/UMass SCGE Protocol for mTmG reporter mice Protocol for intrastriatal injection, dissection and fixation, and immunostaining for GFP expression after activation in mTmG reporter mouse Jax-Sontheimer SCGE protocol for mTmG reporter mice.pdf 21000000034