Experiment Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Experiment Condition S10, Cas9 Assay Description: Quantitation of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. Tissue Measured: epithelium of bronchiole Measurement Type: Editing Efficiency Record ID 15000000278 Editor Alt-R™ S.p. Cas9 Nuclease V3 Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide g-loxP2_C9 Vector Model Species Mouse Model Name Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo Sample Age 8-10 weeks Application Method nasal instillation Application Site airway epithelium Dosage [S10]: 40 µM; [Cas9]: 1.33 µM; [gRNA]: 2 µM Injection Frequency once Injection Rate daily for 2 consecutive days Injection Volume 50 microliters Days post injection 7 days Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description cilia (α-tubulin); nuclei (DAPI) Measured Values Samples Size: 5   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 8.90  1 13.7  2 9.8  3 3.4  4 9.8  5 7.8