RNP-NC-CPP | SCGE Toolkit

SCGE ID	12000000188
Name	RNP-NC-CPP
Description	The nanocapsule is a thin glutathione (GSH)-cleavable covalently crosslinked polymer coating around a preassembled ribonucleoprotein (RNP) complex between a Cas9 nuclease and an sgRNA. This nanoparticle has an addition of a cell penetrating peptide (CPP) from the TAT peptide (GRKKRRQRRRPQ) which lacks cell-type specficity
Type	Nanoparticle
Subtype	polymer-RNP nanocapsule

Source	Gong Lab

Nanoparticle Size	34 nm
Zeta Potential	3 mV

This Delivery System: RNP-NC-CPP is being used ...

Project	Initiative	Contact PI
Enabling Nanoplatforms for Targeted In Vivo Delivery of CRISPR/Cas9 Riboncleoproteins in the Brain Experiments Enabling Nanoplatforms for Targeted in vivo Delivery of CRISPR/Cas9 Ribonucleoproteins in the Brain.	Delivery Systems	Shaoqin (Sarah) Gong, PhD (University of Wisconsin-Madison)
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain	Animal Reporter and Testing Center	Stephen A Murray, PhD (Jackson Laboratory)
Comparing the efficiency and cellular impact of non-viral, hepatocyte-targeted delivery vehicles with viral-mediated gene delivery in a microphysiological liver on chip platform Experiments Testing different ratios of Lipofectamine-RNPs after 24 hours for determination of positive control Quantification of transfection efficiency by flow cytometry with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on liver-on-chip model system Imaging quantification of transfection efficiency with varying dosages of nanoparticles encapsulated with Cas9/sgRNA RNP on the liver-on-chip model system	Collaborative Opportunity Fund	Ross C. Wilson, PhD Samira Kiani, PhD Shaoqin (Sarah) Gong, PhD (University of Pittsburgh)

Delivery System: RNP-NC-CPP