Validation Experiment: Independent validation of Gong delivery platform using RNP-loaded nanocages to deliver CRISPR/Cas9 to mouse brain
PI: Stephen A Murray, PhD
Description: Delivery of CRISPR/Cas9 via RNP-loaded nanocages to the brain in Ai14 mice
Editing Assay:
Editing efficiency calculated by counting the number of Edited Neurons (NeuN+/GFP+ and TdTomato+/RFP+) out of the total number of neurons (NeuN+/GFP+) in a defined region of interest in the brain, expressed as a percentage of neurons in the ROI.
Parent Project: The Jackson Laboratory Gene Editing Testing Center - Gong Validation (Gong, Shaoqin)
Download: Submitted files
Original Experiment/s that are being validated:
Organ System Overview
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