Experiment Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Experiment Condition Arm 1: 306-O12B LNP containing Cas9 mRNA and sgRNA Assay Description: Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei. Tissue Measured: base of cochlear canal Measurement Type: Editing Efficiency Record ID 15000001300 Editor CleanCap® Cas9 mRNA (5moU) Delivery System 306-O12B Delivery System Subtype Nanoparticle Guide sg298 Vector Model Species Mouse Model Name B6.Cg-Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze/J Sample Sex Mixed Application Method canalostomy injection Application Site inner ear Dosage Injection Frequency once Injection Rate Injection Volume 1 microliter Days post injection 6 days Editor Format Lipid Nanoparticle Antidote Id Antidote Description Measured Values Samples Size: 6   Replicate Result (Editing Efficiency) Measurement Units: Signal Mean 1 of 6 Present  1 Present  2 absent  3 absent  4 absent  5 absent  6 absent