sg298 | SCGE Toolkit

SCGE ID	10000000246
Lab ID	sg298
Source	Synthego
Target Locus	Ai9/Ai14 reporter transgene
Species	transgene
Description	This sgRNA targets the Ai9 and related transgenes at multiple sites. 2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases
Guide Compatibility	SpyCas9

Target Sequence	AAGTAAAACCTCTACAAATG
Target Sequence + PAM	AAGTAAAACCTCTACAAATGTGG

Spacer Sequence	AAGUAAAACCUCUACAAAUG
Spacer Length	20
Modifications	2'-O-Methyl at 3 first and last bases, 3' phosphorothioate bonds between first 3 and last 2 bases

This Guide: sg298 is being used ...

Project	Initiative	Contact PI
The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) Experiments Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear	Animal Reporter and Testing Center Initiative	Stephen A Murray, PhD (Jackson Laboratory)

Guide: sg298