Contact SCGE
Your email
Message
Send
Development System
Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear | SCGE Toolkit
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
SCGE Consortium Home
|
About SCGE
|
Contact Us
|
Citing the Toolkit
|
License
Examples:
Epithelium
,
CRISPR
,
AAV
,
Ai9
Login
Home
Explore Data
(current)
Explore Projects
(current)
Genome Editors
(current)
Model Systems
Delivery Systems
Guides
Vectors
Protocols
Home
/
Project
Experiment: Second site validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
PI:
Stephen A Murray, PhD
Parent Project Page
Download Submitted files
Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice
Editing Assay:
-
Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei.
Organ System Overview
View Experimental Details
Connective Tissue
Reproductive
Renal/Urinary
Endocrine
Haemolymphoid
Gastrointestinal
Liver and Biliary
Respiratory
Cardiovascular
Musculoskeletal
Integumentary
Nervous
Sensory
Hematopoietic
Arm 1: 306-O12B LNP containing Cas9 mRNA and sgRNA
Arm 2: 306-S10 LNP containing Cas9 mRNA and gRNA
Arm 3: uninjected control
Legend:
Delivery Efficiency
Editing Efficiency
Target Tissue
Not Available
Analyze Data Sets Available for this Experiment
View Experimental Details
Sensory
Base of cochlear canal (unspecified)
(TARGET)
Scala tympani (unspecified)
(TARGET)