Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

Validation Experiment: Independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear

PI: Stephen A Murray, PhD>

Description: Delivery of CRISPR/Cas9 via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice

Editing Assay: Confocal Imaging of mouse cochlea, direct fluorescence RFP (tdTomato) compared to Hoechst stained nuclei.

Parent Project: The Jackson Laboratory Gene Editing Testing Center - Chen Validation (Chen, Zheng-Yi)

Download: Submitted files

Original Experiment/s that are being validated:

Tissues

Sensory

Editors

CleanCap® Cas9 mRNA (5moU)

Guide Target Locus

Ai9/Ai14 reporter transgene

Guides

sg298

Delivery Systems

306-O12B

306-S10

Models

Ai14 mouse (congenic)

Sex

Mixed

Independent_validation_of_Chen_delivery_platform_using_LNPs_to_deliver_CRISPR/Cas9_to_mouse_inner_ear_
									Editing Efficiency
Record Id	Condition	Tissue	Sex	Editor	Model	Delivery	Target Locus	Guide	Injection Frequency	Editing Efficiency Signal	Image
15000001300	Arm 1: 306-O12B LNP containing Cas9 mRNA and sgRNA	base of cochlear canal (TARGET)	Mixed	CleanCap® Cas9 mRNA (5moU)	Ai14 mouse (congenic)	306-O12B	Ai9/Ai14 reporter transgene	sg298	once	Present; absent; absent; absent; absent; absent;
15000001301	Arm 1: 306-O12B LNP containing Cas9 mRNA and sgRNA	scala tympani (TARGET)	Mixed	CleanCap® Cas9 mRNA (5moU)	Ai14 mouse (congenic)	306-O12B	Ai9/Ai14 reporter transgene	sg298	once	Present; absent; absent; absent; absent; absent;
15000001302	Arm 2: 306-S10 LNP containing Cas9 mRNA and gRNA	base of cochlear canal (TARGET)	Mixed	CleanCap® Cas9 mRNA (5moU)	Ai14 mouse (congenic)	306-S10	Ai9/Ai14 reporter transgene	sg298	once	absent; absent; absent; absent; absent; absent;
15000001303	Arm 2: 306-S10 LNP containing Cas9 mRNA and gRNA	scala tympani (TARGET)	Mixed	CleanCap® Cas9 mRNA (5moU)	Ai14 mouse (congenic)	306-S10	Ai9/Ai14 reporter transgene	sg298	once	absent; absent; absent; absent; absent; absent;
15000001304	Arm 3: uninjected control	base of cochlear canal (TARGET)	Mixed		Ai14 mouse (congenic)				none	absent; absent;
15000001305	Arm 3: uninjected control	scala tympani (TARGET)	Mixed		Ai14 mouse (congenic)				none	absent; absent;

Independent_validation_of_Chen_delivery_platform_using_LNPs_to_deliver_CRISPR/Cas9_to_mouse_inner_ear_
									Editing Efficiency
Record Id	Condition	Tissue	Sex	Editor	Model	Delivery	Target Locus	Guide	Injection Frequency	Editing Efficiency Signal	Image