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Filters ..
  Tissues
Sensory
  • Base of cochlear canal  (TARGET)
  • Scala tympani  (TARGET)
Editors
 CleanCap® Cas9 mRNA (5moU)
Guide Target Locus
 Ai9/Ai14 reporter transgene
Guides
 sg298
  Delivery Systems
 306-O12B
 306-S10
Models
 Ai14 mouse (congenic)
Sex
 Mixed

Results

Independent_validation_of_Chen_delivery_platform_using_LNPs_to_deliver_CRISPR/Cas9_to_mouse_inner_ear_
Editing Efficiency
Record Id Condition Tissue Sex Editor Model Delivery Target Locus Guide Injection Frequency Editing Efficiency Signal Image
15000001300 Arm 1: 306-O12B LNP containing Cas9 mRNA and sgRNA base of cochlear canal (TARGET) Mixed CleanCap® Cas9 mRNA (5moU) Ai14 mouse (congenic) 306-O12B Ai9/Ai14 reporter transgene sg298 once
15000001301 Arm 1: 306-O12B LNP containing Cas9 mRNA and sgRNA scala tympani (TARGET) Mixed CleanCap® Cas9 mRNA (5moU) Ai14 mouse (congenic) 306-O12B Ai9/Ai14 reporter transgene sg298 once
15000001302 Arm 2: 306-S10 LNP containing Cas9 mRNA and gRNA base of cochlear canal (TARGET) Mixed CleanCap® Cas9 mRNA (5moU) Ai14 mouse (congenic) 306-S10 Ai9/Ai14 reporter transgene sg298 once
15000001303 Arm 2: 306-S10 LNP containing Cas9 mRNA and gRNA scala tympani (TARGET) Mixed CleanCap® Cas9 mRNA (5moU) Ai14 mouse (congenic) 306-S10 Ai9/Ai14 reporter transgene sg298 once
15000001304 Arm 3: uninjected control base of cochlear canal (TARGET) Mixed Ai14 mouse (congenic) none
15000001305 Arm 3: uninjected control scala tympani (TARGET) Mixed Ai14 mouse (congenic) none