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Experiment: Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center

PI:   Erik J Sontheimer, PhD 
 

Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain
Editing Assay: - Editing efficiency determined by immunohistochemical staining for GFP.

Filter Results ..
Tissues
 striatum
Editors
 SpyCas9-3xNLS
  Guide Target Locus
 Dnmt1
 mTmG reporter transgene
  Guides
 STS159 (mTmG; Sp_t2:Sp_c20_mTmG)
 STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1)
Models
 mTmG mouse (congenic)
Sex
 Male

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Results

Testing_preparation_for_validation_at_The_Jackson_Laboratory_Small_Animal_Testing_Center
Editing Efficiency
Record Id Condition Tissue Sex Editor Model Target Locus Guide Injection Frequency Editing Efficiency Signal Image
15000001496 DNMT1-C20/T2 Control striatum Male SpyCas9-3xNLS mTmG mouse (congenic) Dnmt1 STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) once
15000001497 mTmG-C20/T2 striatum Male SpyCas9-3xNLS mTmG mouse (congenic) mTmG reporter transgene STS159 (mTmG; Sp_t2:Sp_c20_mTmG) once

Activation of mTmG reporter in mouse striatum

None provided.