Experiment: Testing preparation for validation at The Jackson Laboratory Small Animal Testing Center
PI: Erik J Sontheimer, PhD| Delivery of chemically modified, phosphorothioate (PS)-stabilized crRNA with chemically modified, PS-stabilized tracrRNA to activate the mTmG reporter in mouse brain |
| Editing Assay: |
- Editing efficiency determined by immunohistochemical staining for GFP. |
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Results |
| Editing Efficiency | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Condition | Tissue | Sex | Editor | Model | Target Locus | Guide | Injection Frequency | Signal | Image |
| DNMT1-C20/T2 Control | striatum | Male | SpyCas9-3xNLS | mTmG mouse (congenic) | Dnmt1 | STS204 (DNMT1; Sp_t2:Sp_c20_Dnmt1) | once |
 |
|
| mTmG-C20/T2 | striatum | Male | SpyCas9-3xNLS | mTmG mouse (congenic) | mTmG reporter transgene | STS159 (mTmG; Sp_t2:Sp_c20_mTmG) | once |
 |
|
| Activation of mTmG reporter in mouse striatum |
 |
None provided. |
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