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Experiment: Validating Traffic Light Reporter 2 (TLR-2) Mouse Model via Germline Editing

PI:   Stephen A Murray, PhD

Description: Heterozygous embryos containing the traffic light reporter 2 (TLR-2) reporter at the Rosa26 locus were evaluated for activation potential following electroporation of two guide/RNP combinations and transfer to pseudo-pregnant hosts for development to term. Offspring (founders) with activating edits were mated to wild-type females and adult tissues from progeny were assessed for tagRFP fluorescence.
Editing Assay: Detection of tagRFP fluorescence in tissues collected from adult TLR-2 mice edited through the germline (N1 progeny).
Download: Submitted files
Filters ..
  Tissues
 Brain
 Diaphragm
 Epididymis
 Eye
 Gastrocnemius
 Heart
 Kidney
 Large intestine
 Liver
 Lung
 Lymph node
 Ovary
 Pancreas
 Skin of body
 Small intestine
 Spleen
 Stomach
 Testis
 Thymus
 Urinary bladder
 Uterus
Cell Types
 Unspecified
Editors
 Alt-Râ„¢ S.p. Cas9 Nuclease V3
Guide Target Locus
 Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn
  Guides
 R26-2
 R26-52
Models
 TLR-2 mouse

Results
Validating_Traffic_Light_Reporter_2_(TLR-2)_Mouse_Model_via_Germline_Editing
Editing Efficiency
Record Id Condition Tissue Cell Type Editor Model Target Locus Guide Injection Frequency Editing Efficiency Signal Image
15000005237 Guide 26-2 urinary bladder Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005238 Guide 26-2 brain Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005239 Guide 26-2 diaphragm Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005240 Guide 26-2 eye Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005241 Guide 26-2 gastrocnemius Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005242 Guide 26-2 heart Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005243 Guide 26-2 kidney Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005244 Guide 26-2 large intestine Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005245 Guide 26-2 liver Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005246 Guide 26-2 lung Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005247 Guide 26-2 lymph node Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005248 Guide 26-2 pancreas Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005249 Guide 26-2 skin of body Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005250 Guide 26-2 small intestine Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005251 Guide 26-2 spleen Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005252 Guide 26-2 stomach Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005253 Guide 26-2 thymus Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005254 Guide 26-2 uterus Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005255 Guide 26-2 ovary Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005256 Guide 26-2 epididymis Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005257 Guide 26-2 testis Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-2 once
15000005258 Guide 26-52 urinary bladder Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005259 Guide 26-52 brain Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005260 Guide 26-52 diaphragm Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005261 Guide 26-52 eye Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005262 Guide 26-52 gastrocnemius Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005263 Guide 26-52 heart Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005264 Guide 26-52 kidney Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005265 Guide 26-52 large intestine Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005266 Guide 26-52 liver Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005267 Guide 26-52 lung Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005268 Guide 26-52 lymph node Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005269 Guide 26-52 pancreas Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005270 Guide 26-52 skin of body Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005271 Guide 26-52 small intestine Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005272 Guide 26-52 spleen Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005273 Guide 26-52 stomach Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005274 Guide 26-52 thymus Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005275 Guide 26-52 uterus Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005276 Guide 26-52 ovary Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005277 Guide 26-52 epididymis Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once
15000005278 Guide 26-52 testis Alt-Râ„¢ S.p. Cas9 Nuclease V3 TLR-2 mouse Gt(ROSA)26Sortm12(CAG-Venus*,-TagRFP*)Rkuhn R26-52 once