Validation Experiment: Repeat experiment of independent validation of Chen delivery platform using LNPs to deliver CRISPR/Cas9 to mouse inner ear
PI: Stephen A Murray, PhD
Description: Repeat experiment of CRISPR/Cas9 delivery via bioreducible lipid nanoparticles (LNPs) to the inner ear in Ai14 mice. Subset of mice were administered LNP via canalostomy injection. Control mice were admininstered a blank LNP. Tissues were harvested 6 days after LNP administration. On-target editing was assessed by RFP (tdTomato) signal.
Editing Assay:
On-target editing analyzed by confocal imaging of whole mouse cochlea of direct fluorescence RFP (tdTomato) compared to Hoeschst stained nuclei.
Parent Project: The Jackson Laboratory Gene Editing Testing Center (JAX-GETC) - SATC-Chen, Submission 2 (On- and Off-target data)
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Other experiments in this project: 1
Download: Submitted files
Original Experiment/s that are being validated:
Results |
| Editing Efficiency | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Condition | Tissue | Sex | Editor | Model | Delivery | Target Locus | Guide | Injection Frequency | Signal | Image |
| 306-O12B | base of cochlear canal (TARGET) | CleanCap® Cas9 mRNA (5moU) | Ai14 mouse (congenic) | 306-O12B | Ai9/Ai14 reporter transgene | sg298 | once | |||
| 306-O12B | scala tympani (TARGET) | CleanCap® Cas9 mRNA (5moU) | Ai14 mouse (congenic) | 306-O12B | Ai9/Ai14 reporter transgene | sg298 | once | |||
| Control | base of cochlear canal (TARGET) | Male | Ai14 mouse (congenic) | 306-O12B blank | once | |||||
| Control | scala tympani (TARGET) | Male | Ai14 mouse (congenic) | 306-O12B blank | once | |||||
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