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 sNLS-SpCas9-sNLS
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 Negative control gRNA
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 RNP-NC-CPP
 RNP-NC-no ligand
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 RNP-NC-tri(GalNAc)
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 Liver On Chip (LoC)
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Results

Quantification_of_transfection_efficiency_by_flow_cytometry_with_varying_dosages_of_nanoparticles_encapsulated_with_Cas9/sgRNA_RNP_on_liver-on-chip_model_system
Delivery Efficiency
Record Id Condition Editor Model Delivery Guide Dosage Delivery Efficiency % uptake Image
15000003507 2.4µg RNP-NC Lipo 1:1 sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-no ligand with Lipofectamine 2000 Negative control gRNA 2.4µg RNP
15000003508 2.4µg RNP-NC sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-no ligand Negative control gRNA 2.4µg RNP
15000003509 2.4µg RNP-NC-TAT sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-CPP Negative control gRNA 2.4µg RNP
15000003510 2.4µg RNP-NC-tri(GalNAc) sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-tri(GalNAc) Negative control gRNA 2.4µg RNP
15000003511 24µg RNP-NC Lipo 1:1 sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-no ligand with Lipofectamine 2000 Negative control gRNA 24µg RNP
15000003512 24µg RNP-NC sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-no ligand Negative control gRNA 24µg RNP
15000003513 24µg RNP-NC-TAT sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-CPP Negative control gRNA 24µg RNP
15000003514 24µg RNP-NC-tri(GalNAc) sNLS-SpCas9-sNLS Liver On Chip (LoC) RNP-NC-tri(GalNAc) Negative control gRNA 24µg RNP

Higher dose (24ug) of RNP-NCs shows higher uptake as compared to a lower dose (2.4ug)

A-D shows histogram and cellular uptake of RNP-NC, RNP-NC-TAT, RNP-NC-TriGalNac and lipofectamine-mediated RNP-NC-delivery respectively at the lower dose of 2.4ug. E-F shows histogram and cellular uptake of RNP-NC, RNP-NC-TAT, RNP-NC-TriGalNac and lipofectamine-mediated RNP-NC-delivery respectively at the higher dose of 24ug

A) shows differential uptake by different RNP-NC tagged with different ligands, B) shows tables demonstrating the quantitation of the uptake, C) shows albumin secretion on day 1, and D) shows albumin secretion 18 hrs post RNP-NC addition