Experiment Shuttle peptides enable in vivo gene editing with Cas9 and Cas12a RNP in mouse airway epithelia Experiment Condition S10, Cas12a Assay Description: Quantitation of GFP+ cells in large and small airways following 1 delivery of GFP protein by GFP positive cells compared to DAPI stained cells. Tissue Measured: epithelium of bronchiole Measurement Type: Editing Efficiency Record ID 15000000279 Editor AsCas12a (IDT and Feldan Therapeutics) Delivery System S10 Delivery System Subtype Amphiphilic peptide Guide g-loxPbot_C12a Vector Model Species Mouse Model Name Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo Sample Age 8-10 weeks Application Method nasal instillation Application Site airway epithelium Dosage [S10]: 40 µM; [Cas12a): 2.5 µM; [gRNA]: 2 µM Injection Frequency twice Injection Rate daily for 2 consecutive days Injection Volume 50 microliters Days post injection 7 days Editor Format ribonucleoprotein (RNP) Antidote Id Antidote Description cilia (α-tubulin); nuclei (DAPI) Measured Values Samples Size: 3   Replicate Result (Editing Efficiency) Measurement Units: % of cells Mean 9.90  1 7.2  2 10.7  3 11.8