Experiment: Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting B2M locus
PI: Paul B McCray, Jr, MD Alice F Tarantal, PhD David Guay, PhD David R Liu, PhD
Description: Delivery of ABE8e-Cas9 RNP using Shuttle peptides candidates to human airway epithelial cells cultured at the air liquid interface targeting B2M locus. Editing efficiency was assessed using Sanger sequencing.
Editing Assay:
Editing efficiency was assessed using Sanger sequencing
Parent Project: Base Editing in Rhesus Airway Epithelial
-
Other experiments in this project: 6
- Delivery of Cas9 RNP using Shuttle peptide candidates to human airway epithelial cells cultured at the air liquid interface targeting CFTR locus
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas9
- Inhibitory effect of Cas9 RNP alone on S10- or FSDS315-mediated GFP protein delivery to HeLa cells
- Rhesus macaque CCR5 gRNA screening using ABE8e-Cas12a
- Delivery of ABE8e-Cas9 RNP using Shuttle peptide candidates to rhesus monkey airway epithelial cells cultured at the air liquid interface targeting CCR5 locus.
- Amphiphilic Peptides Deliver Base Editor RNPs to Rhesus Monkey Airway
Download: Submitted files
Select experimental variable to highlight records on the chart:
Note: Hover over the bars to view additional information
Results |
| Editing Efficiency | |||||||
|---|---|---|---|---|---|---|---|
| Condition | Editor | Model | Delivery | Target Locus | Guide | % of reads | Image |
| Mock | ABE8e-Cas9 | Primary Airway Epithelia (Human) | B2M | B2M | |||
| FSD301 | ABE8e-Cas9 | Primary Airway Epithelia (Human) | FSD301 | B2M | B2M | ||
| FSD285 | ABE8e-Cas9 | Primary Airway Epithelia (Human) | FSD285 | B2M | B2M | ||
| FSD197 | ABE8e-Cas9 | Primary Airway Epithelia (Human) | FSD197 | B2M | B2M | ||
| S10 | ABE8e-Cas9 | Primary Airway Epithelia (Human) | S10 | B2M | B2M | ||
| FSD237 | ABE8e-Cas9 | Primary Airway Epithelia (Human) | FSD237 | B2M | B2M | ||
| FSD315 | ABE8e-Cas9 | Primary Airway Epithelia (Human) | FSD315 | B2M | B2M | ||
× Close Overview
