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Editors
 SpCas9 (Feldan Therapeutics)
Guide Target Locus
 CFTR
Guides
 Cas9-CFTR-R553X
  Delivery Systems
 FSD315
 S10
Models
 HeLa
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Results

Inhibitory_effect_of_Cas9_RNP_alone_on_S10-_or_FSDS315-mediated_GFP_protein_delivery_to_HeLa_cells
Delivery Efficiency
Record Id Condition Editor Model Delivery Target Locus Guide Dosage Delivery Efficiency % of fluorescent cells Image
15000002984 Mock HeLa
15000002985 GFP alone HeLa GFP protein (10μM)
15000002986 S10 HeLa S10 GFP protein (10μM) + Shuttle peptide (10μM)
15000002987 S10 + Cas9 RNP SpCas9 (Feldan Therapeutics) HeLa S10 CFTR Cas9-CFTR-R553X GFP protein (10μM) + Shuttle peptide (10μM) + Cas9 RNP (2.5 μM Cas9 and 2 μM gRNA)
15000002988 FSD315 HeLa FSD315 GFP protein (10μM) + Shuttle peptide (10μM)
15000002989 FSD315 + Cas9 RNP SpCas9 (Feldan Therapeutics) HeLa FSD315 CFTR Cas9-CFTR-R553X GFP protein (10μM) + Shuttle peptide (10μM) + Cas9 RNP (2.5 μM Cas9 and 2 μM gRNA)