Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells

Experiment: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells

PI: Paul B McCray, Jr, MD>

Description: Delivery of Cas9 RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel

Editing Assay: Calculated editing efficiency by % of NGS reads that showed an indel

Parent Project: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

Other experiments in this project: 3
- Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells
- Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells

Download: Submitted files

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Results

Peptide_Shuttle_optimization_to_deliver_Cas9_RNP_to_human_airway_epithelia_cells
							Editing Efficiency
Record Id	Condition	Editor	Model	Delivery	Target Locus	Guide	Dosage	Editing Efficiency % of reads	Image
15000000704	FS48	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FS48	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.15; 0.33; 1.2; 1.42; 0.76;
15000000705	FS66d6	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FS66d6	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.77; 6.28; 0.24; 0.8; 0.44; 0.6; 2.37; 1.16; 0.0245; 0.0053; 0.00485;
15000000706	FSD112	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD112	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.6; 3.27; 1.44; 1.5; 0.48; 1.32; 1.34; 1.26; 3.49; 0.62; 0.14; 0.07425; 0.0531; 0.05345;
15000000707	FSD114	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD114	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.36; 2.86; 1.14; 5.54; 2.28;
15000000708	FSD114d1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD114d1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.42; 12.56; 7.33; 1.23; 11.72; 1.47;
15000000709	FSD115	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD115	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.79; 4.28; 1.24;
15000000710	FSD115d1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD115d1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.11; 5.62; 8.97; 3.47; 2.94; 4.24;
15000000711	FSD116d1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD116d1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.21; 1.02;
15000000712	FSD117D1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD117D1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.02; 0.84; 1.07;
15000000713	FSD118	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD118	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.53; 0.73; 0.44;
15000000714	FSD121	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD121	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.86; 0.5; 0.94;
15000000715	FSD122	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD122	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.85; 1.02;
15000000716	FSD132	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD132	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.79; 0.7;
15000000717	FSD147	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD147	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.63; 0.13;
15000000718	FSD159	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD159	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.55; 1.31;
15000000719	FSD160	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD160	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.08; 3.08;
15000000720	FSD168	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.1; 0.2; 0.05;
15000000721	FSD168 cyclic	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168 cyclic	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.39; 1.86; 0.16;
15000000722	FSD168 Disul.	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168 Disul.	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.05; 4.62; 0.07;
15000000723	FSD168 Scr.	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168 Scr.	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.0;
15000000724	FSD168d10	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d10	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.72; 0.97; 0.78;
15000000725	FSD168d11	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d11	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.67; 0.76; 1.16;
15000000726	FSD168d12	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d12	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.81; 0.94; 0.4;
15000000727	FSD168d17	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d17	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.19; 11.94; 9.32; 2.35; 2.3; 3.27;
15000000728	FSD168D20	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168D20	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.83; 1.53; 0.51;
15000000729	FSD186	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD186	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.85; 0.73;
15000000730	FSD188	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD188	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.07; 7.05; 5.41;
15000000731	FSD189	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD189	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0; 7.15; 4.84;
15000000732	FSD190	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD190	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.17; 1.3; 6.19;
15000000733	FSD191	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD191	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.32; 1.18; 2.37;
15000000734	FSD193	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD193	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.74; 0.59; 6.46;
15000000735	FSD195	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD195	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.53; 11.63; 10.95; 1.79; 16.24; 7;
15000000736	FSD196	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD196	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.53; 6.85; 5.49;
15000000737	FSD197	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD197	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.83; 5.59; 4.9;
15000000738	FSD199	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD199	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.78; 9.92; 9.26; 1.61; 1.56; 2.76;
15000000739	FSD215	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD215	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.68; 2.37; 2.06;
15000000740	FSD216	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD216	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.42; 0.62; 0.58;
15000000741	FSD222	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD222	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.11; 3.53; 2.63;
15000000742	FSD227	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD227	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.37; 2.2; 1.5;
15000000743	FSD228	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD228	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.81; 4.16; 2.02;
15000000744	FSD234	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD234	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.42; 3.06; 1.5;
15000000745	FSD235	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD235	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.75; 1.84; 3.07;
15000000746	FSD236	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD236	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.31; 7.45; 3.1;
15000000747	FSD237	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD237	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	23.99; 22.1; 17.2; 5.8; 4.78; 3.08; 11.17; 12.38; 6.73; 9.46; 6.9; 14.1050000190734; 13.9600000381469; 7.28999996185302; 5.29; 14.62; 10.91;
15000000748	FSD238	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD238	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	13.54; 13.45; 16.86; 2.72; 6.96; 1.95; 8.1; 5.1; 2.13; 7.56; 3.38;
15000000749	FSD239	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD239	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.54; 0.96; 0.35;
15000000750	FSD240	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD240	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.71; 1.58; 1.31;
15000000751	FSD259	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD259	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.56; 4.51;
15000000752	FSD260	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD260	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.21; 2.48;
15000000753	FSD262	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD262	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.34; 15.51; 15.21; 21.53; 6.65; 6.32; 15.09; 8.18;
15000000754	FSD283	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD283	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.91; 0.82;
15000000755	FSD284	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD284	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.85; 6.99; 5.23; 2.51; 2.91;
15000000756	FSD285	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD285	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.7; 8.08; 8.33; 2.45;
15000000757	FSD286	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD286	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.25; 10.77;
15000000758	FSD287	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD287	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.81; 6.45; 4.58;
15000000759	FSD288	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD288	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.65; 2.2; 1.31;
15000000760	FSD289	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD289	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.43; 0.83; 0.86;
15000000761	FSD291	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD291	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.66; 0.73; 0.51;
15000000762	FSD293	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD293	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.17; 0.07; 0.13;
15000000763	FSD297	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD297	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.81; 4.73; 2.77;
15000000764	FSD301	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD301	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.12; 7.49; 1.97; 1.14; 5.53; 3.2;
15000000765	FSD303	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD303	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.9; 1.61; 1.68;
15000000766	FSD304	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD304	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.76; 1.23; 2.75;
15000000767	FSD305	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD305	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.86; 0.24; 0.57;
15000000768	FSD306	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD306	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.27; 0.19; 0.25;
15000000769	FSD307	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD307	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.48; 1.98; 5.47;
15000000770	FSD308	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD308	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.07; 0.72; 1.23;
15000000771	FSD310	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD310	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.26; 0.63; 1.54;
15000000772	FSD315	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD315	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	33.8; 11.68; 6.72; 18.26; 8.61;
15000000773	FSD316	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD316	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	12.36; 2.95;
15000000774	FSD317	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD317	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.12; 1.71;
15000000775	FSD318	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD318	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.79; 2.89; 0.06; 6.93; 2.41;
15000000776	FSD319	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD319	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.16; 2.34;
15000000777	FSD321	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD321	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.34; 6.81; 2.69; 13.82; 4.9;
15000000778	FSD322	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD322	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.15; 4.92;
15000000779	FSD323	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD323	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.24; 5.6;
15000000780	FSD329	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD329	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.44; 3.61;
15000000781	FSD330	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD330	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.97; 2.05; 1.62;
15000000782	FSD331	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD331	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	12.44; 15.37; 7.3;
15000000783	FSD332	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD332	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.59; 6.44; 3.38;
15000000784	FSD333	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD333	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.63; 10.83; 4.23;
15000000785	FSD334	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD334	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.02; 8.31; 3.6;
15000000786	FSD335	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD335	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.58; 10.97; 5.18;
15000000787	FSD339	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD339	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.72; 10.9; 3.1;
15000000788	FSD341	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD341	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.32; 0.28; 0.13;
15000000789	FSD347	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD347	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.74; 3.07; 0.33;
15000000790	FSD359	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD359	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.0749999992549419; 0.279999993741512; 0.0700000002980232;
15000000791	FSD360	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD360	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.0350000001490116; 0.499999985098839; 0.179999999701977;
15000000792	FSD361	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD361	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.559999987483024; 0.735000014305114; 0.909999996423721;
15000000793	FSD362	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD362	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.05000001192092; 1.47000002861022; 0.810000002384185;
15000000794	FSD363	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD363	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.01999998092651; 4.23000001907348; 1.75999999046325;
15000000795	FSD364	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD364	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.78000003099441; 8.50999975204467; 1.90500003099441;
15000000796	FSD365	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD365	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.229999996721745; 2.52499997615814; 0.634999990463256;
15000000797	FSD366	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD366	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.97500002384185; 3.80499994754791; 2.08500003814697;
15000000798	FSD367	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD367	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.34499996900558; 3.79500007629394; 3.6800000667572;
15000000799	FSD368	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD368	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.810000002384185; 1.0949999988079; 0.770000010728836;
15000000800	FSD57	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.51; 0.44;
15000000801	FSD57d1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.06; 2.37; 1.31;
15000000802	FSD57d3	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d3	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.17; 1.61; 1.07;
15000000803	FSD57d4	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d4	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.73; 1.42; 0.62;
15000000804	FSD57d5	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d5	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.07; 5.39; 1.23;
15000000805	FSD57d6	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d6	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.05; 1.07; 0.92;
15000000806	FSD63	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD63	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.514999985694885; 1.93999999761581; 1.125;
15000000807	FSD63D1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD63D1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.715000003576278; 2.67499995231628; 1.11500000953674;
15000000808	FSD67	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD67	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.33; 0.56;
15000000809	FSD94	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD94	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.15; 0.14; 0.11;
15000000810	FSD95	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD95	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.65; 2.34; 3.24000000953674; 6.27500009536743; 3.61000001430511;
15000000811	FSD96	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD96	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.119999997317791; 1.02500000596046; 0.170000001788139;
15000000812	FSD97	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD97	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.21; 0.41; 0.94;
15000000813	FSX1	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX1	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.49; 0.77; 0.66;
15000000814	FSX2	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX2	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.97; 0.5; 0.66;
15000000815	FSX3	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX3	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.25; 1.35; 0.86;
15000000816	FSX4	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX4	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.26; 1.06; 0.56;
15000000817	FSX5	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX5	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.84; 0.35; 0.37;
15000000818	FSX6	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX6	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.72; 0.77; 0.57;
15000000819	FSX7	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX7	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.94; 1.59; 1.21;
15000000820	FSX8	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX8	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.47; 0.52;
15000000821	S10	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.82; 2.51; 0.83; 0.74; 3.87; 0.8; 3.06; 0.72; 2.47; 0.5; 1.17; 3.71; 2.67; 3.88; 6.47; 3.58; 1.70999997854232; 3.9300000667572; 4.58499991893768; 2.25; 6.11; 3.27;
15000000822	S10 Scr.	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10 Scr.	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0; 3.85; 1.58;
15000000823	S10D	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10D	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.31; 0.44; 0.5;
15000000824	S10-MOD	SpCas9 (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10-MOD	CFTR	McCray_CFTR_Cas9_Guide1	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.09; 1.81; 0.9;

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Experiment: Peptide Shuttle optimization to deliver Cas9 RNP to human airway epithelia cells

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