Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells

Experiment: Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells

PI: Paul B McCray, Jr, MD

Description: Delivery of Cas12a RNP targeting human CFTR in Primary human epithelia cells. Gene editing efficiency was determined by percentage of NGS reads that showed an indel

Editing Assay: Calculated editing efficiency by % of NGS reads that showed an indel

Parent Project: Delivery of CRISPR Ribonucleoproteins to Airway Epithelia Using Novel Amphiphilic Peptides

Other experiments in this project: 5

Download: Submitted files

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Results

Peptide_Shuttle_optimization_to_deliver_Cas12a_RNP_to_human_airway_epithelia_cells
							Editing Efficiency
Record Id	Condition	Editor	Model	Delivery	Target Locus	Guide	Dosage	Editing Efficiency % of reads	Image
15000000825	FSD117D1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD117D1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.02; 14.98; 12.58;
15000000826	FS66d6	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FS66d6	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.5; 1.9; 3.4;
15000000827	FSD112	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD112	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	28.6; 19.7;
15000000828	FSD114	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD114	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	48.2; 27.79; 17.77; 27.01; 30.9; 44.06; 28.48; 29.54; 33.19; 30.91; 28.1; 34.5; 34.56; 40.53; 14.21; 20.23; 0.1895; 21.78; 20.34; 22.9;
15000000829	FSD114d1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD114d1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	29.3; 22.9; 19.6;
15000000830	FSD115	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD115	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	11; 9.4; 5.7;
15000000831	FSD115d1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD115d1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	19.6; 18.6; 16.7;
15000000832	FSD116d1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD116d1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.7; 16.4;
15000000833	FSD118	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD118	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.9; 5.9; 7.3;
15000000834	FSD121	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD121	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	20.64; 20.32; 16.02; 26.55; 19.88; 20.16;
15000000835	FSD122	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD122	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	19.1; 21;
15000000836	FSD132	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD132	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	13.5; 7.3;
15000000837	FSD147	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD147	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.1; 13.4;
15000000838	FSD159	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD159	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	20.2; 9.5;
15000000839	FSD160	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD160	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.9; 11.9;
15000000840	FSD168	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	11.3; 9.1; 9.4;
15000000841	FSD168 cyclic	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168 cyclic	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.6; 4.2; 6.8;
15000000842	FSD168 Disul.	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168 Disul.	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.3; 10.5; 8.5;
15000000843	FSD168 Scr.	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168 Scr.	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.15; 10.63; 5.17;
15000000844	FSD168d10	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d10	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.73; 11.62; 6.02;
15000000845	FSD168d11	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d11	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.33; 6.77; 5.11;
15000000846	FSD168d12	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d12	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	19.2; 15.6; 17.3;
15000000847	FSD168d17	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d17	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	23.1; 5.79; 4.41;
15000000848	FSD168d20	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD168d20	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.8; 7.7; 15.5;
15000000849	FSD186	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD186	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	17.1; 17.1;
15000000850	FSD188	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD188	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	31.4; 29.4; 24.6; 22.68; 18.2; 17.57;
15000000851	FSD189	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD189	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	35; 36.7; 32.2; 22.28; 27.85; 13.68; 20.68; 36.07; 28.27; 26.56; 17.68; 24.73; 0.2936; 24.49;
15000000852	FSD190	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD190	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.8; 20.5; 10.6;
15000000853	FSD191	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD191	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	19.5; 23.1; 22.7;
15000000854	FSD193	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD193	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	19.8; 25.4; 16.2;
15000000855	FSD195	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD195	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	25.3; 21.9; 23.5;
15000000856	FSD196	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD196	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	28.3; 27.3; 25.8; 32.5; 19.72; 24.04;
15000000857	FSD197	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD197	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	39.2; 35.4; 28.3; 11.95; 19.54; 12.14; 11.75; 12.579999923706; 21.8249998092651;
15000000858	FSD199	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD199	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.3; 25.6; 17.1;
15000000859	FSD215	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD215	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.1; 12.27; 8.5;
15000000860	FSD216	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD216	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.6; 9.23; 11.62;
15000000861	FSD222	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD222	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	15.8; 12.85; 11.34;
15000000862	FSD227	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD227	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.6; 13.04; 19.4;
15000000863	FSD228	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD228	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	22.9; 20.75; 23.21;
15000000864	FSD234	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD234	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	24.5; 27.61; 22.73;
15000000865	FSD235	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD235	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.2; 9.18; 8.1;
15000000866	FSD236	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD236	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.1; 16.26; 12.1;
15000000867	FSD237	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD237	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	33.6; 23.32; 29.24; 32.84; 24.64; 20.88;
15000000868	FSD238	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD238	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	24.6; 20.91; 27.95;
15000000869	FSD239	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD239	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	23.7; 20.74; 10.99;
15000000870	FSD240	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD240	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	22.5; 20.14; 10.22;
15000000871	FSD259	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD259	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.82; 16.1; 10.9;
15000000872	FSD260	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD260	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	10.37; 9.4; 8.31;
15000000873	FSD262	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD262	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	20.67; 33.8; 35.83; 12.88; 7.06; 18.69; 32.62; 25.46; 19.99;
15000000874	FSD283	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD283	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.29; 4.83; 4.66;
15000000875	FSD284	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD284	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	23.55; 22.77; 13.9;
15000000876	FSD285	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD285	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	20.4; 31.56; 42.52; 20.25; 19.91; 29.41; 27.63; 26.91;
15000000877	FSD286	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD286	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	23; 25.55; 23.49;
15000000878	FSD287	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD287	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	25.5; 18.01;
15000000879	FSD288	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD288	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	25.8; 19;
15000000880	FSD289	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD289	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.41; 2.9;
15000000881	FSD291	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD291	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.83; 3;
15000000882	FSD293	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD293	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.23; 0.81; 1.1;
15000000883	FSD297	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD297	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	25.02; 18.27;
15000000884	FSD301	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD301	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	31.33; 25.17; 27.63; 22.18; 22.66;
15000000885	FSD303	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD303	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.02; 4.73;
15000000886	FSD304	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD304	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	13.49; 13.84; 23.45; 30.95; 24.96; 17.12;
15000000887	FSD305	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD305	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.25; 4.8; 2.59;
15000000888	FSD306	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD306	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.07; 3.54; 4;
15000000889	FSD307	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD307	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.11; 22.2; 19.59; 34.75; 33.9; 16.14;
15000000890	FSD308	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD308	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	1.58; 5.07; 4.96;
15000000891	FSD310	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD310	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.81; 5.84; 5.25;
15000000892	FSD315	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD315	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	20.78; 27.59; 41.21; 33.42; 30.43;
15000000893	FSD316	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD316	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	17.71; 19.97;
15000000894	FSD317	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD317	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.26; 10.51;
15000000895	FSD318	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD318	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	18.56; 18.14;
15000000896	FSD319	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD319	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	20.7; 18.47;
15000000897	FSD321	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD321	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	21.49; 22.88;
15000000898	FSD322	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD322	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	17.04; 17.98;
15000000899	FSD323	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD323	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	25.27; 18.06;
15000000900	FSD329	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD329	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	18.15; 12.38;
15000000901	FSD330	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD330	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.04; 7.02; 7.58;
15000000902	FSD331	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD331	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	12.56; 16.41; 19.85;
15000000903	FSD332	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD332	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.77; 7.95; 10.57;
15000000904	FSD333	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD333	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.36; 11.84; 16.69;
15000000905	FSD334	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD334	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.48; 7.81; 11.45;
15000000906	FSD335	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD335	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	11.5; 16.28; 19.65;
15000000907	FSD339	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD339	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	6.44; 6.83; 9.22;
15000000908	FSD341	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD341	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.42; 0.12; 0.3;
15000000909	FSD347	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD347	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	3.21; 1.86; 3.44;
15000000910	FSD359	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD359	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.3450002670288; 8.51499986648559; 5.58500003814697;
15000000911	FSD360	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD360	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.25; 10.5099997520446; 8.59999990463256;
15000000912	FSD361	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD361	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.61500000953674; 21.7600002288818; 20.954999923706;
15000000913	FSD362	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD362	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.69499993324279; 18.9099998474121; 16.2999997138977;
15000000914	FSD363	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD363	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	12.829999923706; 32.7150001525878; 28.5999994277954;
15000000915	FSD364	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD364	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	0.430000007152557; 3.42000007629394; 0.279999993741512;
15000000916	FSD365	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD365	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	13.9000000953674; 12.5999999046325; 13.0700001716613;
15000000917	FSD366	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD366	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	13.3200001716613; 25.8450002670288; 23.7399997711181;
15000000918	FSD367	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD367	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.5149998664855; 24.7100000381469; 25.6700000762939;
15000000919	FSD368	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD368	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.1599998474121; 7.67499995231628; 6.25499987602233;
15000000920	FSD57	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	9.7; 4.5;
15000000921	FSD57d1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	34.7; 18.16; 11.26;
15000000922	FSD57d3	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d3	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	11.83; 11.51; 11.29;
15000000923	FSD57d4	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d4	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	8.04; 9.8; 7.45;
15000000924	FSD57d5	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d5	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.32; 15.61; 5.8;
15000000925	FSD57d6	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD57d6	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.9; 10.09; 7.26;
15000000926	FSD63	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD63	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	11.9700002670288; 6.46500015258789; 4.15999996662139;
15000000927	FSD63D1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD63D1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	16.0699996948242; 15.9500002861022; 15.8949995040893;
15000000928	FSD67	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD67	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	19.9; 9.2;
15000000929	FSD94	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD94	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	29.6; 26.2; 25.9; 29.4; 21.8; 12; 20.2; 21.82; 28.1; 21.18; 32.54; 40.95; 0.1898; 18.82; 17.89;
15000000930	FSD95	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD95	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	18.2; 20.5; 14.68; 15.83; 12.79;
15000000931	FSD96	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD96	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	16.704999923706; 11.7950000762939; 10.75;
15000000932	FSD97	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSD97	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	14.1; 11.8; 11.4;
15000000933	FSX1	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX1	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7; 8.06; 6.55;
15000000934	FSX2	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX2	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.98; 3.21; 3.36;
15000000935	FSX3	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX3	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	10.45; 11.2; 16.66;
15000000936	FSX4	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX4	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.27; 7.15; 7.93;
15000000937	FSX5	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX5	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	5.5; 6.33; 2.77;
15000000938	FSX6	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX6	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.61; 5; 6.88;
15000000939	FSX7	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX7	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	25.19; 24.16; 19.44;
15000000940	FSX8	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	FSX8	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.11; 8.63; 6.23;
15000000941	S10	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	31.6; 21; 14.7; 11.5; 3.7; 22.5; 21.9; 27.3; 20.66; 16.68; 8.76; 15.26; 22.55; 13.24; 16.18; 10.7; 6.46; 10.77; 10.85; 15.59; 11.71; 12.7100000381469; 19.829999923706; 25.1200008392333; 29.63; 22.13; 28.69;
15000000942	S10 Scr.	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10 Scr.	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	4.4; 5.5; 4.5;
15000000943	S10D	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10D	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	2.2; 5.1; 2.7; 1.05; 8.35; 4.58; 1.54; 1.94;
15000000944	S10-MOD	AsCas12a (Feldan Therapeutics)	Primary Airway Epithelia (Human)	S10-MOD	CFTR	3849 45-5'	2uM gRNA, 1.33uM Cas12a, 40uM Shuttle	7.9; 13.2; 15.2;

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Experiment: Peptide Shuttle optimization to deliver Cas12a RNP to human airway epithelia cells

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