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Experiment: Cre Recombinase dose escalation study in Ai9 mice

PI:   Aravind Asokan, PhD 

Description: A single stranded cmv cre cassette was packaged into AAV9 or AAVcc47 and injected intravenously in Ai9 mice. We injected n=3 at three different doses (1e10, 1e11, 1e12 vg) and harvested organs 4 weeks post injection. Fluorescence intensity in liver, heart, and skeletal muscle was quantified with tiff based images in Image J and neuronal transduction from each vector was quantified at the 1e12vg dose by counting the number of tdTomato+ neurons and number of NeuN+ cells from multiple sections and images.
Delivery Assays:
  • Delivery efficency was calculated by measuring fluorescent intensity in tdTomato+ cells from multiple images and sections in liver, heart, skeletal muscle
  • Delivery efficency in brain was calculated by counting the total number of TdTomato+ neurons and total number of NeuN+ cells
Parent Project: Evolving High Potency AAV Vectors for Neuromuscular Genome Editing
Download: Submitted files
Filter Results ..
  Tissues
Liver and Biliary
  • Liver  (TARGET)
Cardiovascular
  • Heart  (TARGET)
Musculoskeletal
  • Skeletal muscle tissue
Nervous
  • Cerebellum (neuron)
  • Hippocampal formation (neuron)
  • Cerebral cortex (neuron)
Cell Types
 neuron
Delivery Systems
 AAV
Models
 Ai9 mouse
  Sex
 Female
 Male
  Vectors
 AAV9-CMV-Cre
 AAVcc47-CMV-Cre
Select experimental variable to highlight records on the chart:

Note: Hover over the bars to view additional information

Results
Cre_Recombinase_dose_escalation_study_in_Ai9_mice_
Delivery Efficiency
Record Id Condition Tissue Cell Type Sex Model Delivery Vector Dosage Injection Frequency Delivery Efficiency Corrected total cell fluorescence Delivery Efficiency % of cells Image
15000000955 AAV9-CMV-Cre, 1e12vg, Male liver (TARGET) Male Ai9 mouse AAV AAV9-CMV-Cre 1e12vg once
15000000956 AAV9-CMV-Cre, 1e12vg, Male heart (TARGET) Male Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000957 AAV9-CMV-Cre, 1e12vg, Male skeletal muscle tissue Male Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000958 AAV9-CMV-Cre, 1e12vg, Male cerebellum neuron Male Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000959 AAV9-CMV-Cre, 1e12vg, Male hippocampal formation neuron Male Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000960 AAV9-CMV-Cre, 1e12vg, Male cerebral cortex neuron Male Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000961 AAV9-CMV-Cre, 1e11vg, Male liver (TARGET) Male Ai9 mouse AAV9-CMV-Cre 1e11vg once
15000000962 AAV9-CMV-Cre, 1e11vg, Male heart (TARGET) Male Ai9 mouse AAV9-CMV-Cre 1e11vg once
15000000963 AAV9-CMV-Cre, 1e11vg, Male skeletal muscle tissue Male Ai9 mouse AAV9-CMV-Cre 1e11vg once
15000000964 AAV9-CMV-Cre, 1e10vg, Male liver (TARGET) Male Ai9 mouse AAV9-CMV-Cre 1e10vg once
15000000965 AAV9-CMV-Cre, 1e10vg, Male heart (TARGET) Male Ai9 mouse AAV9-CMV-Cre 1e10vg once
15000000966 AAV9-CMV-Cre, 1e10vg, Male skeletal muscle tissue Male Ai9 mouse AAV9-CMV-Cre 1e10vg once
15000000967 AAV9-CMV-Cre, 1e12vg, Female liver (TARGET) Female Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000968 AAV9-CMV-Cre, 1e12vg, Female heart (TARGET) Female Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000969 AAV9-CMV-Cre, 1e12vg, Female skeletal muscle tissue Female Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000970 AAV9-CMV-Cre, 1e12vg, Female cerebellum neuron Female Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000971 AAV9-CMV-Cre, 1e12vg, Female hippocampal formation neuron Female Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000972 AAV9-CMV-Cre, 1e12vg, Female cerebral cortex neuron Female Ai9 mouse AAV9-CMV-Cre 1e12vg once
15000000973 AAV9-CMV-Cre, 1e10vg, Female heart (TARGET) Female Ai9 mouse AAV9-CMV-Cre 1e10vg once
15000000974 AAVcc47-CMV-Cre, 1e12vg, Male liver (TARGET) Male Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000975 AAVcc47-CMV-Cre, 1e12vg, Male heart (TARGET) Male Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000976 AAVcc47-CMV-Cre, 1e12vg, Male skeletal muscle tissue Male Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000977 AAVcc47-CMV-Cre, 1e12vg, Male cerebellum neuron Male Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000978 AAVcc47-CMV-Cre, 1e12vg, Male hippocampal formation neuron Male Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000979 AAVcc47-CMV-Cre, 1e12vg, Male cerebral cortex neuron Male Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000980 AAVcc47-CMV-Cre, 1e11vg, Male liver (TARGET) Male Ai9 mouse AAVcc47-CMV-Cre 1e11vg once
15000000981 AAVcc47-CMV-Cre, 1e11vg, Male heart (TARGET) Male Ai9 mouse AAVcc47-CMV-Cre 1e11vg once
15000000982 AAVcc47-CMV-Cre, 1e11vg, Male skeletal muscle tissue Male Ai9 mouse AAVcc47-CMV-Cre 1e11vg once
15000000983 AAVcc47-CMV-Cre, 1e10vg, Male liver (TARGET) Male Ai9 mouse AAVcc47-CMV-Cre 1e10vg once
15000000984 AAVcc47-CMV-Cre, 1e10vg, Male heart (TARGET) Male Ai9 mouse AAVcc47-CMV-Cre 1e10vg once
15000000985 AAVcc47-CMV-Cre, 1e10vg, Male skeletal muscle tissue Male Ai9 mouse AAVcc47-CMV-Cre 1e10vg once
15000000986 AAVcc47-CMV-Cre, 1e12vg, Female liver (TARGET) Female Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000987 AAVcc47-CMV-Cre, 1e12vg, Female heart (TARGET) Female Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000988 AAVcc47-CMV-Cre, 1e12vg, Female skeletal muscle tissue Female Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000989 AAVcc47-CMV-Cre, 1e12vg, Female cerebellum neuron Female Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000990 AAVcc47-CMV-Cre, 1e12vg, Female hippocampal formation neuron Female Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000991 AAVcc47-CMV-Cre, 1e12vg, Female cerebral cortex neuron Female Ai9 mouse AAVcc47-CMV-Cre 1e12vg once
15000000992 AAVcc47-CMV-Cre, 1e10vg, Female heart (TARGET) Female Ai9 mouse AAVcc47-CMV-Cre 1e10vg once
15000000993 Mock, 1e12vg, Male liver (TARGET) Male Ai9 mouse
15000000994 Mock, 1e12vg, Male heart (TARGET) Male Ai9 mouse
15000000995 Mock, 1e12vg, Male skeletal muscle tissue Male Ai9 mouse
15000000996 Mock, 1e12vg, Male cerebellum neuron Male Ai9 mouse
15000000997 Mock, 1e12vg, Male hippocampal formation neuron Male Ai9 mouse
15000000998 Mock, 1e12vg, Male cerebral cortex neuron Male Ai9 mouse
15000000999 Mock, 1e11vg, Male liver (TARGET) Male Ai9 mouse
15000001000 Mock, 1e11vg, Male heart (TARGET) Male Ai9 mouse
15000001001 Mock, 1e11vg, Male skeletal muscle tissue Male Ai9 mouse
15000001002 Mock, 1e10vg, Male liver (TARGET) Male Ai9 mouse
15000001003 Mock, 1e10vg, Male heart (TARGET) Male Ai9 mouse
15000001004 Mock, 1e10vg, Male skeletal muscle tissue Male Ai9 mouse